10x User Guides, Support home Universal 5' Gene Expression Documentation Library Construction Chromium X Series User Guide with Readiness Test Chromium Single Cell 5' Reagent Kits User Guide (v2 - Next-GEM) with Feature Barcoding technology for Cell Surface Protein and Immune Receptor Mapping CG000772 Stepwise Objectives The Chromium Single Cell Gene Expression Solution upgrades short read sequencers to deliver a scalable microfluidic platform for 3ʹ digital gene expression by profiling 500 For additional guidance, refer to the User Guides cited below: For guidance on qualifying the Chromium X, refer to the Chromium X Series Specifications (CG000415). pdf View and download file Chromium Next GEM Single Cell 3’ Reagent 10x Genomics Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing Stepwise Objectives Kits Chromium Single Cell Multiome ATAC + Gene Expression Reagent Kits User Guide About us Investors Careers Contact News Distributors View and Download 10x Genomics Chromium X Series user manual online. Consult the relevant assay-specific user guide on the 10x Genomics Support website for the list of compatible kits and consumables. BFX: Tools and Tutorials for 10x Genomics Applications The table below lists 10x Genomics single-cell and spatial applications, the associated software tools (with official website/support links), and Support home Universal 3' Gene Expression Documentation Library Construction Chromium Single Cell 3' Reagent Kits User Guide (v3. For Single Cell 3' Gene Expression library construction, View and Download 10x Genomics Visium CytAssist user manual online. To start the test Additional types of readiness tests available on the instrument are only to be launched by 10x Field Engineers. 0" are compatible with Visium HD 6. 5 mm projects, please use original protocol 500-10,000 cells per channel 500-30,000 cells per channel with CellPlex • Next GEM HT: 2,000–20,000 cells per channel 2,000–60,000 cells per channel with CellPlex Consult the relevant assay-specific View and Download 10x Genomics Visium CytAssist user manual online. Chromium X Series Quick Reference Cards User Guide, CG000471 CG000471_ChromiumX-QuickReferenceCards_RevH. Support home Universal 5' Gene Expression Documentation Instruments Chromium X Series Chromium X Series User Guide with Readiness Test Visium Spatial Gene Expression Reagent Kits User Guide User Guide, CG000239 CG000239_VisiumSpatialGeneExpression_UserGuide_RevH. Xenium In Situ – Protocol 10x Genomics Account Onscreen instruction will guide account setup. For ongoing Visium HD 6. Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Bundle, 4 rxns PN-1000285, includes: Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Bundle, 4 rxns PN-1000285, includes: Find Product User Guide for Targeted Gene Expression Find instrument user guide Sort by Most popular Latest chemistry CG000293 All 10x Genomics Next GEM single cell assays are supported on Chromium X Series instruments. General guidance for library storage, quality control Chromium Next GEM Training Kit User Guide User Guide, CG000210 CG000210_ChromiumNextGEMTrainingKitUserGuide_RevG. com to create an account beforehand. Chromium X Series laboratory equipment pdf manual download. pdf View and download file What does 10x Genomics Cloud Analysis cost to use? Cloud Analysis provides free limits that allow all users to process data on a per-sample basis. It provides a step-by-step guide covering all aspects of the workflow, In the event of an instrument return to 10x Genomics, Support will guide the user through the correct documentation, in addition to the packaging procedure below. English 中文 日本語 usly using the relevant 10x Genomics User Guides. Also for: Chromium x, Chromium ix, Chromium xo, Pn Select an instrument to find user guide for your specific 10x Genomics instrument. , user manuals, operating guides & specifications View and Download 10x Genomics Chromium X Series user manual online. 10xgenomics. Xenium Analyzer User Guide User Guide, CG000584 CG000584_Xenium_Analyzer_UserGuide_RevK. Introduction With our 10x Genomics Single Cell Gene Expression Flex service, we offer complete, scalable solutions for measuring single-cell gene expression in formaldehyde-fixed samples. Xenium Workflow Document Resources Experimental Planning Before starting, refer to these documents for protocol planning and site preparation for installation. Visium CytAssist laboratory equipment pdf manual download. For guidance on sample preparation for library construction and sequencing, refer to the applicable Demonstrated Protocol and User Guide available at the 10x Genomics Support website. Key companion documents for use with this document: Visium HD 3' Spatial Gene Expression cdn. This document provides step-by-step instructions for installation, first-time use, readiness 10x Genomics Pre-designed Gene Panels Visit the 10x Genomics Support website for the most updated information regarding all the available pre-designed panels. View and Download 10x Genomics Chromium X Series user manual online. View online or download the 10x Genomics Visium CytAssist Quick Reference guide for instrument operation. It provides a step-by-step guide covering all aspects of the workflow, The 10x Multiome user guide serves as the definitive resource for researchers working with this groundbreaking technology. pdf View and Assay Prepare tissue sections on Xenium slides for on-instrument analysis by following our step-by-step User Guide/Protocols. Users run "Readiness Test". Each uploaded 10x Genomics library comes with a set Library Construction Construct a sequencing-ready single cell library by following our step-by-step User Guide / Protocol. View and Download 10x Genomics Visium CytAssist user manual online. Instrument serial number and instrument The Chromium Connect offers a fully-integrated automated system, which enables users to run the 10x Genomics library construction workflow end-to-end with minimal user interaction. This tutorial uses the 10x Genomics Cloud Analysis platform to preform the raw data processing for a set of human whole blood lysis with the intent to explore neutrophil maturation. Also for: 1000441, The 10x Multiome user guide serves as the definitive resource for researchers working with this groundbreaking technology. First-time setup (listed below) describes Follow the guidelines in the relevant automation workflow user guides (listed below) for quantification of the cDNA to be used in library construction. Consult the Chromium X Series (X/iX) User Guide (CG000396) for detailed instrument operation instructions and follow the Chromium X touchscreen prompts for execution. pdf View and download file 10x Genomics Account Onscreen instruction will guide account setup. The following section provides guidance on sample preparation Consult the relevant assay-specific user guide on the 10x Genomics Support website for the list of compatible kits and consumables. Instrument serial number and instrument Support home Universal 3' Gene Expression Documentation Library Construction Chromium Single Cell 3' Reagent Kits User Guide (v3. For generating Targeted Gene Expression libraries from Visium Spatial Gene Expression libraries, consult the Spatial Targeted Gene Expre Emulsion-safe Plastics • Use 10x Genomics validated emulsion-safe plastic consumables when handling GEMs as some plastics can destabilize GEMs. Key companion documents for 10x Genomics Single Cell protocols require a suspension of viable single cells or nuclei as input. This user guide Sample Preparation This User Guide can be used for both gene expression only and concurrent gene & protein expression worflows. We have 2 10x Genomics Chromium iX Series manuals available for free PDF download: User Manual, Quick Start Manual Explore the steps for library preparation in Universal 3' Gene Expression with detailed guides and resources to enhance your research. 5 mm and 11 mm slides and contain a "Featured" tag. See below for the documents needed to prepare samples for this User Guide. Alternatively, visit cloud. Also for: View and Download 10x Genomics Chromium X Series user manual online. pdf View and download 10x Genomics Find Product User Guide for Epi Multiome ATAC + Gene Expression Find instrument user guide Sort by Most popular Latest chemistry CG001689 Provides guidance on generating Visium HD 3' libraries from fresh frozen tissue sections. This document outlines the protocol for generating Visium HD Spatial Gene Expression libraries from FFPE, Fresh Frozen, and Fixed Frozen tissue sections. Chromium Single Cell 5' Reagent Kits User Guide (v2 - Next-GEM) with Feature Barcoding technology for Cell Surface Protein and Immune Receptor Mapping CG000772 The Library houses 10x Genomics Product Literature, Posters, Application Notes, and Research Snapshots. User Guides Find step-by-step user guides and protocols for your specific 10x Genomics product. 1 Chemistry). com User Guide | Chromium X Series Instrument | Introduction IntroductionThe Stepwise Objectives The Chromium GEM-X Single Cell Gene Expression v4 upgrades short read sequencers to deliver a scalable microfluidic platform for 3' digital gene expression by profiling 500 Chromium Single Cell 3' Reagent Kits User Guide (v3. For guidance on sample preparation ests will appear. This user guide details the instrument safety and compliance information, system components, system Introduction Introduction Product Identification Product Specifications 6 CG000396 | Rev K Link to TOC | 10xgenomics. Consult GEM-X Chromium Single Cell 5' Reagent Kits User Guide (v2 Chemistry Dual Index) User Guide, CG000331 CG000331_ChromiumNextGEMSingleCell5-v2_UserGuide_RevF. Also for: Chromium x, Chromium ix, Chromium xo, Pn User Guides Protocols labeled "2. pdf View and download file FOR USE Chromium X Series User Guide with Readiness Test User Guide, CG000396 CG000396_ChromiumX_Instrument_UserGuide_Rev_N. com © 2026 10x Genomics. pdf View and download file User Guide, CG000471 Sample Preparation This User Guide is compatible with samples prepared in tubes as well as in 96-well plates. Additional Kits, Reagents & Equipment The items in the table below have been validated by 10x Genomics® and are highly recommended for Single Cell 3’ workflow, training and system View and Download 10x Genomics Chromium X Series user manual online. pdf View and download file Support home Universal 3' Gene Expression Documentation Instruments Chromium X Series Chromium X Series User Guide with Readiness Test Visium CytAssist Spatial Gene and Protein Expression Reagent Kits Company About us Investors Careers Contact News Distributors Find Product User Guide for HD Spatial Gene Expression Find instrument user guide Sort by Most popular Latest chemistry CG000685 For use with: Chromium GEM-X Single Cell 3' Kit v4, 16 rxns PN-1000691 | 4 rxns PN-1000686 Chromium GEM-X Single Cell 3' Chip Kit v4, 4 chips PN-1000690 Dual Index Kit TT Set A, 10x Genomics Account Onscreen instruction will guide account setup. 1 - Next-GEM) with Feature Barcoding technology for Cell Surface Protein CG000316 Chromium X Series User Guide with Readiness Test Provides a detailed overview of the system and usage instructions for Chromium X series. 1 Chemistry Dual Index) Chromium X Series User Guide with Readiness Test User Guide, CG000396 CG000396_ChromiumX_Instrument_UserGuide_Rev_N. All Rights Reserved. pdf View and download file Question: What sequencing configurations are used for 10x Genomics libraries? Answer: Recommended sequencing configurations can be found in: The sequencing section of each assay User Guide, which Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) 10x Genomics Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing This document describes best practices and provides guidance for sequencing 10x Genomics libraries to maximize data output. Manuals and User Guides for 10x Genomics Chromium iX Series. Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) 10x Genomics Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Kits User Guide User Guide, CG000338 CG000338_ChromiumNextGEM_Multiome_ATAC_GEX_User Chromium GEM-X Single Cell 5' v3 Gene Expression User Guide User Guide, CG000733 CG000733_ChromiumGEM-X_SingleCell5_ReagentKitsv3_UserGuide_RevA. Also for: Cg000542. Chromium Single Cell 5' HT Reagent Kits User Guide (v2 - Next-GEM) with Feature Barcode technology for Cell Surface Protein and Immune Receptor Mapping CG000511 Visium CytAssist Spatial Gene and Protein Expression Reagent Kits Company About us Investors Careers Contact News Distributors This user guide details the instrument safety and compliance information, system components, system operation, and troubleshooting guidance. Minimizing the presence of cellular aggregates, dead cells, noncellular nucleic acids, and For use with: GEM-X Training Reagents, Gel Beads and Chip Kit 16 rxns PN-1000745 Chromium GEM-X Single Cell 3' Chip Kit 4 chips PN-1000690 GEM-X OCM Training Reagents, Gel Refer to the Visium Spatial Gene Expression Reagent Kits – Tissue Optimization User Guide (CG000238) for the complete protocol for optimizing permeabilization time for any tissue of interest. Also for: Xenium Analyzer User Guide User Guide, CG000584 CG000584_Xenium_Analyzer_UserGuide_RevK. User Guides Filter user guides Chemistry Version v2 - GEM-X / Apex (3) v1 - GEM-X (4) Xenium In Situ Gene Expression with Cell Segmentation Staining User Guide CG000760 View & download of more than 45 10x Genomics PDF user manuals, service manuals, operating guides. These documents span across all areas of interest, product, instrument, and application Consult the Chromium X Series (X/iX) User Guide (CG000396) for detailed instrument operation instructions and follow the Chromium X touchscreen prompts for execution. 10X Genomics – Guide to Getting Started This page is intended to familiarize Gene Expression Center clients with the many aspects of the various 10X Genomics single cell/single Emulsion-safe Plastics • Use 10x Genomics validated emulsion-safe plastic consumables when handling GEMs as some plastics can destabilize GEMs. Select a product to find user guide for your specific 10x Genomics product. yihh, 21my2, aswwdhmy, fwu8cf, hv, wz9be8, ia1w, v4lym, dtla, xoz, \